DSPE-PEG-SH to DSPE-PEG-CONHNH2
September 9th, 2016
Present: Lily, Tyra, Ina
Task: Synthesis of DSPE-PEG-CONHNH2
- Added 1 mL DSPE-PEG-SH (in PBS) to 3.5 mg MPBH (in 1 mL PBS)
- Weight of MBPH: 36 mg
- Separated into two tubes, in each of the tubes we added 500 microlitres DSPE-PEG-SH and 500 microlitres MPBH
- Left in cold room to be taken out next day
September 10th, 2016
Present: Lily, Tyra
Task: Dialysis of DSPE-PEG-CONHNH2
- Let tubes sit out in RT for 1 hour and then began dialysis with dH2O
Trial 2 with new DSPE-PEG-Amine
September 18, 2016
Present: Lily
Task: Synthesis of DSPE-PEG-CONHNH2 (trial 2)
Weight of tube: 0.8969 g
Weight of DSPE-PEG-SH: 12.7 mg
- Resuspended 12.7 mg DSPE-PEG-SH in 2.535 mL PBS for a concentration of around 1.6 mM
- Set aside the following from DSPE-PEG-SH in PBS:
- 1 uL DSPE-PEG-SH + 500 uL PBS for DLS
- 500 uL set aside for thiol assay
- 1000 uL used for synthesis steps (see below)
- Remainder in tube labelled “DPC” in blue can use for NMR or second thiol assay
MPBH Reaction:
- Added 1000 uL of DSPE-PEG-SH in PBS to 1000 uL MPBH (3.5 mg/1 mL PBS) and allowed for 4 hours incubation in RT
- Began dialysis using 1 kDa dialysis tubing