Synthesis of APOE


Micelle Transfer

October 5, 2016
Present: Edward, Lily, Ina, Amir

Task: Combined APOE/Liposome to EGF/Liposome and APOE/HZ/Lipid to EGF/Liposome through the micelle transfer method

Methodology

Incubated in water bath that varied from 66-64 centrigrade for ~ 1 hour

Dialysis at ~900x volume excess PBS buffer for 2 hours

Dialysis at ~900x volume excess PBS buffer overnight

Liposome Protocol