Micelle Transfer
October 5, 2016
Present: Edward, Lily, Ina, Amir
Task: Combined APOE/Liposome to EGF/Liposome and APOE/HZ/Lipid to EGF/Liposome through the micelle transfer method
Methodology
- Pipetted 117.8uL of Nathan’s APOE/Liposome compound into a micro centrifuge tube
- labelled tube as MT Nathan (in blue sharpie)
- added 117.8uL of EGF/Liposome complex
- Pipetted 478uL of Lily’s APOE/HZ/Lipid compound into a micro centrifuge tube
- labelled tube as MT Lily (in black sharpie)
- added 31.9uL of EGF/Liposome complex
Incubated in water bath that varied from 66-64 centrigrade for ~ 1 hour
Dialysis at ~900x volume excess PBS buffer for 2 hours
Dialysis at ~900x volume excess PBS buffer overnight