When discussing our theme this year, all of us had a common idea to play a game with DNA Origami. That was the beginning of this project. Here, we would like to use DNA origami to play games which are distinguishable like card games. In the case of playing cards, it is necessary to distinguish 52 cards except the jokers. To detect DNA origami, there are various ways such as AFM, TEM, labelling and so on. Labelling with fluorescence was chosen this time due to the high sensitivity and the possibility to be observed with our naked eyes. By incorporating the three primary fluorophores into DNA origami, we will be able to play games and hopefully this could be applied in other areas such as biosensing fields.
To color and distinguish DNA origami, many fluorophores are required and it would be expensive. Then, we got a clue from TV to solve the cost problem by RGB which consist of three primary colors which able to make different colors. Our concept is to make white fluorescent based on RGB system by using red, green and blue fluorophores. To make various colors, it is important to consider the mix proportion of these fluorophores. As demonstrated in the overview of our project, we are able to fabricate DNA Origami structure with various colors of fluorophores. To incorporate fluorophores into DNA origami, we use extended staple DNA which is complement to the sequence of fluorophore modified DNA. Temperature cascading program enables the staple DNA to anneal to the M13mp18ssDNA to form DNA origami decorated with flourophores. The end product is observed with AFM and fluorescence spectrophotometer.
Our goal is to make color distinguishable DNA origami structures based on RGB by using fluorescence spectrophotometer. If we can do that, a lot of DNA origami structures with other colors could be made due to the system of RGB.
OVER VIEW OF OUR PROJECT
In this project, five steps are going to be taken as the below figure shows.
① To measure the fluorocence intensity of three kinds of fluorophores respectively.
② To simulate the mix proportion of the fluorophores on a PC application.
③ To mix each fluorophore and make colors, following the mix propotion.
④ To synthesize the fluorophore modified DNA complement to the extended staple DNA.
⑤ To fabricate and observe the DNA Origami structure by AFM and fluorescence spectrophotometer.
Team Name: Team Kansai
Institution Name: Kansai University
Geographic Location: Osaka, Japan