(experimental procedure)
    3’-Amino modifier C7 modified 10 mer DNA which has the sequence shown below (5’-ACT CGT GCA G-3’-(CH2)7-NH2) was synthesized. After washing the inside of the column with methanol and drying under reduced pressure, the CPG-DNA was deprotected with TCA/DCM. It was heated at 65℃ for 10 mins for the deprotection of phosphate group and nucleic acid base and cleavage from the CPG support at the same time. After drying again, the DNA, 7-hydroxy-4-methylcoumarin-3-acetic acid and DMT-MM were added into an Eppendorf tube, dissolved in DMSO and stirred over night. Thereafter, it was purified by NAP column and HPLC and identified by MALDI-TOF/MS.

Theoretical value=3,543.0 g/mol      ε=95,400

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STEP4-2 OVERVIEW STEP5

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Team Name: Team Kansai
Institution Name: Kansai University
Geographic Location: Osaka, Japan