(experimental procedure)
3’-Amino modifier C7 modified 10 mer DNA which has the sequence shown below (5’-GTG CTT TCG G -3’-(CH2)7-NH2) was synthesized. After washing the inside of the column with methanol and drying under reduced pressure, the CPG-DNA was deprotected with TCA/DCM. It was transferred to a screw cap tube and it was heated at 65℃ for 10 mins for the deprotection of phosphate group and nucleic acid base and cleavage from the CPG support at the same time. After drying again, the DNA, ATTO647N and DMT-MM were added into an Eppendorf tube, dissolved in DMSO and stirred over night. The solution was transferred to a CPG column and washed with acetonitrile. Thereafter, it was purified by NAP column and HPLC and identified by MALDI-TOF/MS.

Theoretical value=3,827.0 g/mol ε=88,600

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STEP4 OVERVIEW STEP4-2

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Team Name: Team Kansai
Institution Name: Kansai University
Geographic Location: Osaka, Japan