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    It has been reported that the background of intact vertebrate embryos of the zebra fish was chased by recognizing specific mRNA with the fluorescence probe consisting of DNA and fluorophore(1). We believe that our colored DNA origami can also be applied to that, considering it as the fluorescence probe in the scale of DNA origami structure. Moreover, in our case, more kinds of fluorescence probes are available because desired colors can be represented with only three fluorophores based on RGB. Therefore, more targets could be chased with our DNA origami structure. As it is reported in DNA PAINT(2), we might possibly observe the intracellular behavior such as organelles called microtubule and mitochondria by using ultra-resolution fluorescent microscope. In that case, it is expected that we would observe not mixed color but the color of each fluorescent molecule. Hence, we can distinguish some kinds of fluorescence probes by changing the placements of fluorophores on the DNA origami structure.

  (1) Next-Generation in Situ Hybridization Chain Reaction: Higher Gain, Lower Cost, Greater Durability
Harry M. T. Choi, Victor A. Beck, and Niles A. Pierce ACS Nano, 2014, 8(5), pp4284-4294
  (2)Multiplexed 3D Cellular Super-Resolution Imaging with DNA- PAINT and Exchange-PAINT
R. Jungmann, M.S. Avendano, J.B. Woehrstein, M. Dai, W.M. Shih, and P. Yin Nat Methods. 2014 March ; 11(3): 313–318.

Team Name: Team Kansai
Institution Name: Kansai University
Geographic Location: Osaka, Japan