Abstract

Nowadays, faster rapid test becomes urgently needed for emerging spread of viruses in highly mobilized population. Therefore, we developed a novel platform with a combination of molecule-aptamer capturing and electrochemical detection . Aptamer is a short stretch of nucleotides which has high binding affinity and selectivity to their target molecule. We had conjugated the aptamers to magnetic graphenes, which were used as conductive material for electrochemical detection. Magnetic force was used to extract aptamer-graphene complexes for further purification. When the target molecule was captured by aptamer, it induced a fast and significant change in electrical resistance that can be easily distinguished. Taken together, we assumed that this platform serves as a novel tool for molecular detection with high sensitivity and specificity, time- and cost-saving, as well as simplified operation for clinical application. Furthermore, we will optimize this platform and its operating procedure to make it commercially feasible.

Introduction

The application of electrochemistry in detection makes the general public easier to understand their health. Due to the high sensitivity of this technique of detection, it requires just small amount of samples. Besides, the outcome is quickly demonstrated in numerical data. It enables the general public simply manipulate the device and interpret the result. The blood glucose meters is such a successful model of the idea which change the diabetes patients’ life. The device has the advantages which has mentioned above that it makes the patients easier to monitor their blood glucose level. Our imagination is sparked by the blood glucose meters. We are ambitious to use electrochemistry to detect the pathogens to make the detection feasible to everyone.

The goal is making our device affordable to everyone to understand their own circumstance of health. Thus, the cost is our top priority. We use aptamers to recognize the pathogens [1], instead of antibody, because aptamer is much cheaper than antibody. Additionally, the graphene is used as the conductive carrier, which can carry the aptamer to the target molecule [2]. When the aptamer-modified graphene binds to the pathogen the increase of electrical resistance of pathogen-aptamer-modified graphene will be soon detected by electrochemistry.

The property of carboxylated graphene [3], as a conductive sensor, has been tested by several ways. First, we conjugated different concentration of tyrosine to determine the sensitivity of carboxylated graphene. Then, the antibody is conjugated to carboxylated graphene to test the outcome of antibody-antigen interaction by the rise of electrical resistance [2]. Hereafter, the aptamer is synthesized on carboxylated graphene to capture the virus.

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References

[1] Feng X, Liu K, Ning Y, Chen L, Deng L, A Label-free Aptasensor for Rapid Detection of H1N1 Virus based on Graphene Oxide and Polymeraseaided Signal Amplification, J Nanomed Nanotechnol, 6 (3), (2015)

[2] Hung-Wei Yang, Chih-Wen Lin, Mu-Yi Hua, Combined Detection of Cancer Cells and a Tumor Biomarker by an Immunomagnetic Sensor to Improve Prostate Cancer Diagnosis, Advanced Materials, 26 (22), 3662–3666 (2014)

[3] Hai-Chen Wu, Xueling Chang, Lei Liu, Feng Zhao and Yuliang Zhao, J. Mater. Chem., Chemistry of carbon nanotubes in biomedical applications, 20, 1036-1052 (2010)