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Lab Book
Cell Culture
Maintenance Log for U251
Maintenance Log for b.End 3
Mini Experiment 1: Seeding Density Titration for 24 Well Plate
Mini Experiment 2: Cellomics Trial Run
Mini Experiment 3: Liposome Compatibility with Well Plates
Experiment 1: Effect of EGF Ligands on Endocytosis Through U251 Cells
Mini Experiment 4: Seeding Titration Experiment on Transwell Filters and Porosity Test
Experiment 2: Fluorescence Permeability Assays
Experiment 3: Analysis of APOE's Effect on Transcytosis Through b.END3 Cells
Experiment 3 Continued: APOE Transcytosis Assay and Full System Analysis
DLS Sizing
All DLS sizing
Synthesis of DSPE
DSPE-PEG-Amine to DSPE-PEG-SH
DSPE-PEG-SH to DSPE-PEG-CONHNH2
Thiol Assay
TNBSA Assay
MALDI-MS
Periodate Oxidation of APOE
Synthesis of EGF
Preparation of Buffers
Reconstitution of EGF
Thiolation of EGF and Conjugation to Liposome
Protein Assays
Maleimide/Thiol Assay
Synthesis of APOE
Micelle Transfer
APOE-only Liposome/APOE + EGF Liposome Protocol
Models
Team
ubcbiomodteam
biomod2016/ubc
ubcbiomod@gmail.com
UBC BIOMOD © 2016
